Journal: Bioactive Materials

Article Title: Nanozyme hydrogels remodel pathological microenvironment for temporomandibular joint osteoarthritis therapy via inhibiting MAPK signal pathway

doi: 10.1016/j.bioactmat.2026.01.031

Figure Lengend Snippet: The in vivo therapeutic effect of Mn-NC composite hydrogels on TMJ-OA after 2 weeks. (a) Schematic diagram of in vivo experiments of hydrogel treatment of TMJ-OA. HE staining (b), SO/FG staining (c), immunohistochemical staining of COLII (d) and MMP13 (e), immunofluorescence staining of CD86 (f) and CD206 (g) at the synovial, and immunofluorescence staining of IL-1β (h) in the TMJ of rats after 2 weeks of treatment, scale bar = 100 μm. (i) Histological OARSI score of articular cartilage after 2 weeks of treatment and quantitative data of immunohistochemical positive area and proportion of immunofluorescence positive cells in (c–h). Data represent means ± SD, n = 3. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 indicates a significant difference in comparison to the Sham group. # p < 0.05, ## p < 0.01, and ### p < 0.001 suggests a significant difference in comparison to the MIA group.

Article Snippet: Additionally, the sections were stained with antibodies specific to anti-CD86, CD206, and IL-1β (Boster Biological Technology Co., Ltd., Wuhan, China) for immunofluorescence analysis.

Techniques: In Vivo, Staining, Immunohistochemical staining, Immunofluorescence, Comparison

Journal: Bioactive Materials

Article Title: Nanozyme hydrogels remodel pathological microenvironment for temporomandibular joint osteoarthritis therapy via inhibiting MAPK signal pathway

doi: 10.1016/j.bioactmat.2026.01.031

Figure Lengend Snippet: The in vivo therapeutic effect of Mn-NC composite hydrogels on TMJ-OA after 8 weeks. HE staining (a), SO/FG staining (b), immunohistochemical staining of COLII (c) and MMP13 (d), immunofluorescence staining of CD86 (e) and CD206 (f) at the synovial, and immunofluorescence staining of IL-1β (g) in the TMJ of rats after 8 weeks of treatment, scale bar = 100 μm. (h) Histological OARSI score of articular cartilage after 8 weeks of treatment and quantitative data of immunohistochemical positive area and proportion of immunofluorescence positive cells in (b–g). Data represent means ± SD, n = 3. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 indicates a significant difference in comparison to the Sham group. # p < 0.05, ## p < 0.01, and ### p < 0.001 suggests a significant difference in comparison to the MIA group.

Article Snippet: Additionally, the sections were stained with antibodies specific to anti-CD86, CD206, and IL-1β (Boster Biological Technology Co., Ltd., Wuhan, China) for immunofluorescence analysis.

Techniques: In Vivo, Staining, Immunohistochemical staining, Immunofluorescence, Comparison

Journal: BioMed Research International

Article Title: Effect of Wnt Signaling on the Differentiation of Islet β -Cells from Adipose-Derived Stem Cells

doi: 10.1155/2017/2501578

Figure Lengend Snippet: Immunostaining of rat ADSCs, rat ADSCs induced into insulin-producing cells, and rat PASCs. (a–e) CD13, CD44, CD49d, CD106, and control group (no primary antibody) staining of rat ADSCs. (f–j) PDX1, nestin, CK19, insulin, and control group staining of rat ADSCs induced into insulin-producing cells. (k–o) PDX1, nestin, CK19, insulin, and control group staining of rat PASCs. Magnification: ×100.

Article Snippet: CD13 (BA0718), CD44 (BA0321), CD106 (BA0406), and CD49d (BA1640) antibodies were from Boster Biological (Wuhan, China).

Techniques: Immunostaining, Control, Staining

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Photobiomodulation mitigates chondrocyte catabolism in osteoarthritis by modulating macrophage M1 polarization through the IL-6/JAK/STAT pathway

doi: 10.1186/s13018-025-06506-4

Figure Lengend Snippet: The IF staining and the representative micro-CT images of mouse knees. A The effects of CLD Lips and PBM intervention on macrophage depletion by IF staining ( n = 4). F4/80 is a pan-macrophage marker (green). B The effects of CLD Lips and PBM intervention on macrophage polarization by IF staining ( n = 4). CD86 is a M1 macrophage marker (green), and CD206 is a M2 macrophage marker (red). C 2D reconstruction of micro-CT images of knee joints. D BV/TV and E Tb.Sp of micro-CT analysis ( n = 5). (Data are presented as mean ± SD; Statistical significance was determined by one-way ANOVA followed by Tukey’s post-hoc test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

Article Snippet: After cooling and blocking, sections were incubated overnight at 4 °C with the second primary antibody, anti-CD206 antibody (Cat. No. A02285-2, rabbit polyclonal, Bosterbio, USA) diluted 1:100.

Techniques: Staining, Micro-CT, Marker

Journal: International Journal of Medical Sciences

Article Title: RBM15 activates glycolysis in M1-type macrophages to promote the progression of aortic aneurysm and dissection

doi: 10.7150/ijms.97185

Figure Lengend Snippet: Immunofluorescence staining of vascular tissue. (A) Immunofluorescence staining graph of M1 subtype of vascular tissues in AD and normal groups, blue (DAPI, nucleus) and green (iNOS), (B) Staining statistics graph. (C) Immunofluorescence staining graph of vascular tissue M2 subtype in AD and normal groups, red (CD206), (D) M2 subtype staining statistic graph. (* p < 0.05; ** p < 0.01).

Article Snippet: Primary antibodies, including CD206 antibody (Lot: YA798, MCE, USA) and iNOS antibody (Lot: HY-P80725, MCE, USA), were applied, followed by incubation with secondary antibodies, specifically goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody conjugated with Alexa FluorTM 488 (Lot: A-11034, Thermo Fisher, USA).

Techniques: Immunofluorescence, Staining

Journal: International Journal of Medical Sciences

Article Title: RBM15 activates glycolysis in M1-type macrophages to promote the progression of aortic aneurysm and dissection

doi: 10.7150/ijms.97185

Figure Lengend Snippet: Expression of M1 and M2 type-related markers in arterial macrophages after knockdown of RBM15 in the SD rat model of AD was examined by flow cytometry. (A) Expression of the M1 macrophage marker iNOS and (B) fluorescence intensity analysis of iNOS expression, (C) expression of the M1 macrophage marker CD206 and (D) fluorescence intensity analysis of CD206 expression. (** p < 0.01).

Article Snippet: Primary antibodies, including CD206 antibody (Lot: YA798, MCE, USA) and iNOS antibody (Lot: HY-P80725, MCE, USA), were applied, followed by incubation with secondary antibodies, specifically goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody conjugated with Alexa FluorTM 488 (Lot: A-11034, Thermo Fisher, USA).

Techniques: Expressing, Knockdown, Flow Cytometry, Marker, Fluorescence